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PubMed DOI Seiwerth S, Sikiric P, Grabarevic Z, et al

It is closed with rubber stopper then an aluminum or plastic cap is placed to avoid contamination and making it tamper-proof

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However, this conformational change reduces the space available to accommodate the -helix of the peptide increasing the steric hindrance between the bulky ionic lock (R17 Cagri- E14 Cagri ) and ECL2